4-Nitrophenyl-beta-D-glucopyranoside CAS: 2492-87-7

Kuyesa kwa enzyme: Hydrolysis ya 4-Nitrophenyl-beta-D-glucopyranoside ndi ma enzymes ngati β-glucosidase angagwiritsidwe ntchito kuyeza ntchito ya enzyme.Kutulutsidwa kwa 4-nitrophenol kumatha kuwerengedwa pogwiritsa ntchito spectrophotometry, kulola kutsimikiza kwa enzyme kinetics ndi maphunziro oletsa.

Mafotokozedwe a jini ndi ma assays atolankhani: 4-Nitrophenyl-beta-D-glucopyranoside imatha kuphatikizidwa ndi ma tag apadera a maselo omwe amaphatikizidwa mu mapuloteni ophatikizika.Ntchito ya ma enzyme ya puloteni yosakanikirana imatha kuwerengedwa mosavuta poyesa kutulutsidwa kwa 4-nitrophenol, kupereka chidziwitso pakulankhula kwa jini ndi kuyanjana kwa mapuloteni ndi mapuloteni.

Kupezeka kwa mankhwala osokoneza bongo komanso kuwunika kwapamwamba: Hydrolysis ya 4-Nitrophenyl-beta-D-glucopyranoside ingagwiritsidwe ntchito ngati njira yoyeserera pofufuza mankhwala.Zimalola kuwunika mwachangu kwamalaibulale akulu akulu kuti adziwe zoletsa ma enzyme kapena ma activator.

Kuyezetsa matenda: Matenda ena amalumikizidwa ndi kusintha kwa zochitika zina za ma enzyme.Pogwiritsa ntchito hydrolysis ya 4-Nitrophenyl-beta-D-glucopyranoside ndi michere yapadera monga kuyesa kwa matenda, matendawa amatha kupezeka kapena kuyang'aniridwa.Izi zikuphatikiza mikhalidwe yokhudzana ndi zovuta zosungirako za lysosomal ndi matenda a hepatobiliary.