BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine mchere CAS:6578-06-9

BCIP-Toluidine, yomwe imadziwikanso kuti mchere wa 5-Bromo-4-chloro-3-indolylphosphate-p-toluidine, ndi mankhwala omwe amagwiritsidwa ntchito kwambiri mu kafukufuku wa zamoyo zama molekyulu ndi biochemistry. Amagwiritsidwa ntchito makamaka ngati gawo la kafukufuku wa enzyme-linked immunosorbent assays (ELISAs) kapena njira zoyeretsera za immunohistochemical kuti azindikire ntchito ya alkaline phosphatase.

Pamene BCIP-Toluidine yadulidwa ndi alkaline phosphatase, imadutsa mu kusintha kwa mtundu, zomwe zimapangitsa kuti pakhale buluu-wofiirira. Izi zimathandiza kuti pakhale kuwonetsa ndi kuwerengera ntchito ya alkaline phosphatase m'zitsanzo. Mphamvu ya mtunduwo imatha kuyezedwa pogwiritsa ntchito spectrophotometric ndipo imagwirizana mwachindunji ndi ntchito ya enzyme.

Kugwiritsa ntchito BCIP-Toluidine ngati substrate kumachitika makamaka pakugwiritsa ntchito ma label a DNA kapena RNA, monga in situ hybridization (ISH) kapena Southern and Northern blotting. Kumapereka njira yodalirika komanso yodziwira bwino kupezeka kwa ma nucleic acid omwe ali mu zitsanzo.

Oda kapena Pemphani Mtengo

Tsatanetsatane wa Zamalonda

Ma tag a Zamalonda

Kugwiritsa Ntchito ndi Zotsatira zake

BCIP-Toluidine (mchere wa 5-Bromo-4-chloro-3-indolylphosphate-p-toluidine) ndi chinthu chomwe chimagwiritsidwa ntchito mu njira za biology kuti chizindikire kukhalapo kwa alkaline phosphatase. Ikagwiritsidwa ntchito ngati chinthu choyambira, BCIP-Toluidine imakumana ndi kusintha kwa mtundu ikagawanika ndi alkaline phosphatase, zomwe zimapangitsa kuti pakhale mtundu wabuluu-wofiirira. Kusintha kwa mtundu kumeneku kumathandiza ofufuza kuzindikira ndikuwona kuchuluka kwa alkaline phosphatase m'mitundu yosiyanasiyana ya mayeso.

Kugwiritsa ntchito kwakukulu kwa BCIP-Toluidine kuli mu njira zoyesera za enzyme-linked immunosorbent (ELISAs) ndi njira zoyezera immunohistochemical. Nthawi zambiri imagwiritsidwa ntchito kuzindikira ma antibodies a alkaline phosphatase-conjugated kapena ma probe a alkaline phosphatase-labeled mu mayeso awa. BCIP-Toluidine imagwiritsidwa ntchito kwambiri mu kafukufuku wa biology ya mamolekyulu kuti iwonetse ndi kuwerengera momwe majini amagwirira ntchito, malo a mapuloteni, ndi ntchito ya enzyme.

Kulongedza Zinthu:

Zina Zowonjezera:

Kapangidwe kake	C8H6BrClNO4P.C7H9N
Kuyesa	99%
Maonekedwe	Ufa woyera
Nambala ya CAS	6578-06-9
Kulongedza	Kakang'ono komanso kochuluka
Moyo wa Shelufu	zaka 2
Malo Osungirako	Sungani pamalo ozizira komanso ouma
Chitsimikizo	ISO.