DEPC CAS:1609-47-8 Mtengo wa Wopanga

Diethyl pyrocarbonate (DEPC) ndi mankhwala omwe amagwiritsidwa ntchito kwambiri m'ma laboratories a biology. Ndi madzi owoneka bwino komanso opanda mtundu omwe amagwira ntchito kwambiri ku ma enzyme a nucleophilic monga amines ndi thiols. DEPC imagwiritsidwa ntchito makamaka kuletsa ma RNases (ribonucleases), ma enzyme omwe amatha kuwononga RNA, mwa kusintha malo awo ogwirira ntchito. Imakwaniritsa izi mwa kuchitapo kanthu ndi zotsalira za histidine mu enzyme, zomwe ndizofunikira pakugwira ntchito kwake. Izi zimapangitsa DEPC kukhala chida chamtengo wapatali muzoyesa zokhudzana ndi RNA ndikuwonetsetsa kuti RNA imasungidwa bwino. Kuphatikiza pa zinthu zake zoletsa RNase, DEPC imatha kusinthanso ma biomolecule ena, monga mapuloteni ndi DNA, chifukwa cha kuchitapo kanthu kwake ndi magulu a nucleophilic. Muyenera kusamala mukamagwira ntchito ndi DEPC, chifukwa ndi poizoni komanso ikhoza kusintha.

Oda kapena Pemphani Mtengo

Tsatanetsatane wa Zamalonda

Ma tag a Zamalonda

Kugwiritsa Ntchito ndi Zotsatira zake

Kuletsa kwa Ma RNases: Cholinga chachikulu cha DEPC ndikuletsa ma RNases, omwe ndi ma enzyme omwe amatha kuwononga RNA. DEPC imagwira ntchito ndi zotsalira za histidine mu ma RNases, kusintha malo awo ogwira ntchito ndikupangitsa kuti asagwire ntchito. Izi zimatsimikizira kusungidwa kwa umphumphu wa RNA panthawi yoyesera monga kusungunula RNA, kusindikizanso kwa reverse transcription, ndi kutsata kwa RNA.

Kusunga zitsanzo za RNA: Chithandizo cha DEPC nthawi zambiri chimagwiritsidwa ntchito pazida za labotale, monga machubu a microcentrifuge, ma pipette tips, ndi madzi omwe amagwiritsidwa ntchito mu kuyesa kwa RNA. Izi zimathandiza kuletsa ma RNases otsala omwe angakhalepo ndikuletsa kuwonongeka kwa RNA panthawi yosamalira ndi kusunga zitsanzo.

Kukonzekera kwa reagent yopanda RNAse: DEPC ingagwiritsidwe ntchito pochiza mayankho kapena ma reagent omwe amafunika kukhala opanda RNAse, monga ma buffers, mayankho a Tris-EDTA (TE), kapena madzi omwe amagwiritsidwa ntchito mu mayesero a biology ya mamolekyulu. Mayankho ochiritsidwawa angagwiritsidwe ntchito mosamala kuti achepetse kuwonongeka kwa RNA.

Kusintha kwa DNA: DEPC imathanso kuchitapo kanthu ndi magulu a amino m'mamolekyu a DNA, zomwe zimapangitsa kuti maziko a DNA asinthidwe ndi carbethoxylated. Izi zitha kugwiritsidwa ntchito pazinthu zinazake, monga DNA sequencing reactions kapena kusintha DNA kuti iphunzire za crosslinking.

Kulongedza Zinthu:

Zina Zowonjezera:

Kapangidwe kake	C6H10O5
Kuyesa	99%
Maonekedwe	Madzi opanda mtundu
Nambala ya CAS	1609-47-8
Kulongedza	Kakang'ono komanso kochuluka
Moyo wa Shelufu	zaka 2
Malo Osungirako	Sungani pamalo ozizira komanso ouma
Chitsimikizo	ISO.