Diethylene triamine pentaacetic acid CAS:67-43-6

Kuletsa kwa Ma RNases: Cholinga chachikulu cha DEPC ndikuletsa ma RNases, omwe ndi ma enzyme omwe amatha kuwononga RNA. DEPC imagwira ntchito ndi zotsalira za histidine mu ma RNases, kusintha malo awo ogwira ntchito ndikupangitsa kuti asagwire ntchito. Izi zimatsimikizira kusungidwa kwa umphumphu wa RNA panthawi yoyesera monga kusungunula RNA, kusindikizanso kwa reverse transcription, ndi kutsata kwa RNA.

Kusunga zitsanzo za RNA: Chithandizo cha DEPC nthawi zambiri chimagwiritsidwa ntchito pazida za labotale, monga machubu a microcentrifuge, ma pipette tips, ndi madzi omwe amagwiritsidwa ntchito mu kuyesa kwa RNA. Izi zimathandiza kuletsa ma RNases otsala omwe angakhalepo ndikuletsa kuwonongeka kwa RNA panthawi yosamalira ndi kusunga zitsanzo.

Kukonzekera kwa reagent yopanda RNAse: DEPC ingagwiritsidwe ntchito pochiza mayankho kapena ma reagent omwe amafunika kukhala opanda RNAse, monga ma buffers, mayankho a Tris-EDTA (TE), kapena madzi omwe amagwiritsidwa ntchito mu mayesero a biology ya mamolekyulu. Mayankho ochiritsidwawa angagwiritsidwe ntchito mosamala kuti achepetse kuwonongeka kwa RNA.

Kusintha kwa DNA: DEPC imathanso kuchitapo kanthu ndi magulu a amino m'mamolekyu a DNA, zomwe zimapangitsa kuti maziko a DNA asinthidwe ndi carbethoxylated. Izi zitha kugwiritsidwa ntchito pazinthu zinazake, monga DNA sequencing reactions kapena kusintha DNA kuti iphunzire za crosslinking.