Disodium 4-nitrophenylphosphate CAS:4264-83-9

Zotsatira zazikulu ndi kugwiritsa ntchito kwa disodium 4-nitrophenylphosphate ndikugwiritsa ntchito kwake ngati gawo la chromogenic poyesa alkaline phosphatase enzyme. Pamene mankhwalawa aphatikizidwa ndi alkaline phosphatase, enzyme imadula gulu la phosphate kuchokera ku disodium 4-nitrophenylphosphate, zomwe zimapangitsa kuti 4-nitrophenol ipangidwe.

4-nitrophenol yomwe imapangidwa ndi phula lachikasu lomwe limatha kuzindikirika ndi spectrophotometric pa 405 nm. Kusintha kwa mtundu kumeneku kumagwiritsidwa ntchito poyesa kuchuluka kwa ntchito ya alkaline phosphatase m'zitsanzo, monga madzi achilengedwe kapena ma cell lysates.

Kugwiritsa ntchito disodium 4-nitrophenylphosphate poyesa ma enzyme kumapereka njira yosavuta yoyezera ntchito ya alkaline phosphatase. Enzyme iyi imagwira ntchito m'njira zosiyanasiyana zamoyo ndipo nthawi zambiri imagwiritsidwa ntchito ngati chizindikiro cha kusiyana kwa minofu, ntchito ya chiwindi, ndi kagayidwe ka mafupa.

Kuphatikiza apo, disodium 4-nitrophenylphosphate ingagwiritsidwe ntchito ngati gawo la ma phosphatase ena, monga acid phosphatase kapena protein phosphatase, kutengera momwe zinthu zakhazikitsidwira komanso cholinga cha kafukufuku.