FLUORESCEIN MONO-BETA-D- GALACTOPYRANOSIDE CAS:102286-67-9

Fluorescein mono-beta-D-galactopyranoside, yomwe imadziwikanso kuti FMG, ndi gulu la fulorosenti lomwe limagwiritsidwa ntchito ngati gawo laling'ono pamayesero osiyanasiyana a biochemical ndi cell biology.Amachokera ku methyl-beta-D-galactopyranoside poyigwirizanitsa ndi molekyulu ya fluorescein.FMG imagwiritsidwa ntchito kwambiri pophunzira ntchito ya beta-galactosidase, puloteni yomwe imayambitsa hydrolysis ya lactose mu galactose ndi shuga.Pogwiritsa ntchito FMG ngati gawo laling'ono, ofufuza amatha kuyang'anira ntchito ya enzymatic ya beta-galactosidase kudzera mu kuyeza kwa fluorescence emission.Hydrolysis ya FMG yopangidwa ndi beta-galactosidase imatsogolera kutulutsa kwa fluorescein, zomwe zimapangitsa kuwonjezeka kwa siginecha ya fulorosenti yomwe imatha kuwerengedwa.FMG ingagwiritsidwe ntchito ngati kafukufuku wa mamolekyu kuti aphunzire kuyanjana kwa ma lectin (mapuloteni omwe amamangiriza ku chakudya) ku chakudya chokhala ndi galactose.Kumangirira kwa ma complexes a FMG-lectin kumatha kuzindikirika ndikuwerengedwa motengera kusintha kwa fluorescence emission.Ponseponse, FMG ndi chida chosunthika powerenga zochitika za ma enzyme ndi kuzindikira kwa carbohydrate, yopereka njira yabwino komanso yovuta kuyeza fluorescence ndikuwunika njira zamoyo izi.

Konzani kapena Funsani Mtengo

Tsatanetsatane wa Zamalonda

Zogulitsa Tags

Kugwiritsa Ntchito ndi Zotsatira

Fluorescein mono-beta-D-galactopyranoside (FMG) ndi molekyu yomwe imagwiritsidwa ntchito kwambiri pofufuza zamoyo ngati gawo lapansi kuti azindikire kukhalapo ndi zochita za enzyme ya beta-galactosidase.FMG ndi yochokera ku shuga lactose ndipo imalumikizidwa ndi molekyulu ya fluorescein.

Chotsatira chachikulu cha FMG ndikuti imapangidwa ndi hydrolyzed ndi beta-galactosidase, puloteni yomwe imaphwanya lactose kukhala galactose ndi shuga.Enzymatic hydrolysis iyi ya FMG imatsogolera kutulutsidwa kwa fluorescein, yomwe imatulutsa chizindikiro champhamvu cha fluorescence.

Kugwiritsa ntchito kwakukulu kwa FMG ndikuzindikira ndi kuyeza zochitika za beta-galactosidase mu zitsanzo zosiyanasiyana.Enzyme iyi imapezeka m'zamoyo zambiri, kuphatikiza mabakiteriya ndi ma cell a mammalian, ndipo ntchito yake imatha kuwonetsa njira zosiyanasiyana zama cell ndi njira zama metabolic.

Pogwiritsa ntchito FMG ngati gawo lapansi, ntchito ya beta-galactosidase imatha kuyesedwa poyang'anira fluorescence yotulutsidwa ndi liberated fluorescein.Kuyeza uku kutha kuchitidwa m'mayesero osiyanasiyana, kuphatikiza ma in vitro assays ndi maphunziro oyerekeza a cell.

Kuphatikiza apo, FMG itha kugwiritsidwa ntchito ngati chida chophunzirira kugawa ndi kumasulira kwa beta-galactosidase m'maselo.Pogwiritsa ntchito njira zowonera ma microscopy a fulorosenti, ofufuza amatha kuwona m'maganizo mwawo mawonekedwe a fluorescence opangidwa ndi FMG pa hydrolysis, kuwalola kuti azitha kuyang'anira zochitika zapanthawi ndi nthawi za beta-galactosidase.

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Kupanga	C26H22O10
Kuyesa	99%
Maonekedwe	White ufa
CAS No.	102286-67-9
Kulongedza	Zing'onozing'ono ndi zambiri
Shelf Life	zaka 2
Kusungirako	Sungani pamalo ozizira ndi owuma
Chitsimikizo	ISO.