Nitro Blue Tetrazolium Chloride monohydrate CAS:298-96-4

Redox reaction: NBT chloride monohydrate imadutsa mu redox reaction ikachepetsedwa ndi ma enzyme ena kapena zinthu zina zochepetsera. Izi zimapangitsa kuti pakhale mtundu wa buluu wakuda wa formazan precipitate, zomwe zimasonyeza kukhalapo ndi ntchito ya ma enzyme awa a reductase.

Kuzindikira Enzyme: NBT chloride monohydrate imagwiritsidwa ntchito kwambiri mu mayeso a enzymatic kuti izindikire momwe ma enzyme osiyanasiyana a reductase amagwirira ntchito. Mwachitsanzo, imagwiritsidwa ntchito pozindikira ma dehydrogenase, succinate dehydrogenase, nitroreductases, ndi ma enzyme ena a oxidoreductase. Mtundu wa buluu wa formazan womwe umapangidwa umasonyeza kukhalapo ndi ntchito ya ma enzyme awa, zomwe zimapangitsa kuti ikhale yothandiza pakuwunika momwe ma enzyme amagwirira ntchito komanso kuphunzira za komwe akuchokera.

Kupaka utoto wa histochemical: NBT chloride monohydrate imagwiritsidwa ntchito mu njira zopaka utoto wa histochemical kuti iwonetse kufalikira ndi ntchito ya ma enzyme mkati mwa minofu. Ingagwiritsidwe ntchito kuphunzira malo a ma enzyme m'mitundu inayake ya maselo kapena magawo a minofu, zomwe zimathandiza ofufuza kumvetsetsa momwe ma enzyme amagwirira ntchito komanso momwe amagwirira ntchito mkati mwa zitsanzo zamoyo.

Kuyesa kwa moyo wa maselo: NBT chloride monohydrate imagwiritsidwa ntchito poyesa moyo wa maselo kuti ione momwe kagayidwe kachakudya kamagwirira ntchito komanso momwe maselo alili. Kuchepa kwa NBT chloride kumasonyeza kukhalapo kwa ma enzymes a reductase m'maselo, zomwe zikusonyeza kuti ali ndi moyo wabwino komanso momwe kagayidwe kachakudya kamagwirira ntchito.

Maphunziro a microbiological: NBT chloride monohydrate imagwiritsidwa ntchito mu maphunziro a microbiological kuti izindikire kupezeka kwa ma enzymes enaake a microbial. Chosakaniza ichi chingagwiritsidwe ntchito poyesa momwe ma enzyme ena amapangidwa ndi mabakiteriya kapena bowa amagwirira ntchito, zomwe zimathandiza kuzindikira ndi kuzindikira mitundu ya tizilombo toyambitsa matenda.