ONPG CAS:369-07-3 Mtengo wa Wopanga

O-nitrophenyl-β-D-galactopyranoside (ONPG) ndi chinthu chopangidwa chomwe chimagwiritsidwa ntchito mu mayeso a biochemical ndi molecular biology kuti ayesere ntchito ya enzyme β-galactosidase. Chimagwiritsidwa ntchito kwambiri mu mayeso kuti chizindikire momwe majini amagwirira ntchito m'mabakiteriya, monga Escherichia coli. ONPG ndi chinthu chopanda utoto chomwe chimadulidwa ndi β-galactosidase, zomwe zimapangitsa kuti pakhale chikasu, o-nitrophenol. Mtundu wachikasu womwe umapangidwa ukhoza kuyezedwa ndi spectrophotometric, kupereka muyeso wosalunjika wa ntchito ya enzyme. Kuyesa kogwiritsa ntchito ONPG nthawi zambiri kumatchedwa ONPG assay ndipo ndi njira yogwiritsidwa ntchito kwambiri mu kafukufuku wa biology ya molecular kuti ayese kuchuluka kwa majini omwe amalamulidwa ndi lac operon m'maselo a bakiteriya.

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Kugwiritsa Ntchito ndi Zotsatira zake

Zotsatira za ONPG monga gawo lapansi ziyenera kudulidwa ndi enzyme β-galactosidase, zomwe zimapangitsa kuti chinthu chachikasu chitulutsidwe, o-nitrophenol. Kusintha kwa mtundu kumeneku kungayesedwe mwa spectrophotometric, zomwe zimathandiza kuti ntchito ya β-galactosidase iwerengedwe. Kugwiritsa ntchito ONPG makamaka kumachitika poyesa momwe majini amagwirira ntchito mu sayansi ya zamoyo ndi microbiology. Nthawi zambiri amagwiritsidwa ntchito poyesa ntchito ya β-galactosidase ngati mtolankhani wa maphunziro okhudza majini, makamaka mabakiteriya monga E. coli. Jini la lacZ, lomwe limapanga β-galactosidase, nthawi zambiri limagwiritsidwa ntchito ngati chizindikiro cha kusanthula momwe majini amagwirira ntchito, chifukwa momwe amagwirira ntchito amatha kuyambitsidwa ndi zinthu zinazake kapena kulamulidwa ndi othandizira enaake. Kuyesa kwa ONPG kumapereka njira yosavuta komanso yodalirika yowunikira kuchuluka kwa momwe majini amagwirira ntchito poyesa ntchito ya β-galactosidase. Kuyesa kumeneku kumagwiritsidwa ntchito kwambiri m'njira zosiyanasiyana, monga kuphunzira momwe majini amagwirira ntchito, malamulo a majini, ndi kuyanjana kwa mapuloteni. Kuphatikiza apo, ingagwiritsidwe ntchito kudziwa momwe ma enzyme amagwirira ntchito ndikuwunika zotsatira za kusintha kwa majini kapena mankhwala pa ntchito ya enzyme.

Chitsanzo cha Zamalonda

Kulongedza Zinthu:

Zina Zowonjezera:

Kapangidwe kake	C12H15NO8
Kuyesa	99%
Maonekedwe	Ufa woyera
Nambala ya CAS	369-07-3
Kulongedza	Kakang'ono komanso kochuluka
Moyo wa Shelufu	zaka 2
Malo Osungirako	Sungani pamalo ozizira komanso ouma
Chitsimikizo	ISO.