ONPG CAS: 369-07-3 Mtengo Wopanga

Zotsatira za ONPG ngati gawo lapansi ziyenera kung'ambika ndi enzyme β-galactosidase, zomwe zimapangitsa kutulutsidwa kwa chinthu chachikasu, o-nitrophenol.Kusintha kwa mtundu uku kungathe kuyeza spectrophotometrically, kulola kuchulukitsidwa kwa ntchito ya β-galactosidase.Kugwiritsira ntchito ONPG makamaka pakuwunika kwa gene expression mu biology ya molekyulu ndi kafukufuku wa microbiology.Nthawi zambiri amagwiritsidwa ntchito poyeza zochita za β-galactosidase monga mtolankhani wa maphunziro a jini, makamaka mabakiteriya monga E. coli.Jini la lacZ, lomwe limayika β-galactosidase, nthawi zambiri limagwiritsidwa ntchito ngati cholembera pakuwunika kwa jini, chifukwa mawu ake amatha kutengera momwe zinthu ziliri kapena kuyendetsedwa ndi olimbikitsa ena. geni poyesa ntchito ya β-galactosidase.Kuyesa uku kumagwiritsidwa ntchito kwambiri m'njira zosiyanasiyana, monga kuphunzira zochitika za olimbikitsa, kuwongolera majini, komanso kuyanjana kwa mapuloteni ndi mapuloteni.Kuphatikiza apo, itha kugwiritsidwa ntchito kudziwa ma enzyme kinetics ndikuwunika zotsatira za masinthidwe kapena mankhwala pakuchita kwa ma enzyme.