Phenylgalactoside CAS: 2818-58-8

Mmene ma enzyme amagwirira ntchito: Phenylgalactoside imagwiritsidwa ntchito poyesa ntchito ya enzyme β-galactosidase.Phenylgalactoside ikakhala hydrolyzed ndi β-galactosidase, imatulutsa p-nitrophenol.Kuchuluka kwa p-nitrophenol kumatha kuyeza mochulukira, kupereka zidziwitso za ntchito ya β-galactosidase.Izi zimagwiritsidwa ntchito ngati ma enzyme assays ndi ma screening system.

Kusanthula kwa ma gene: Phenylgalactoside nthawi zambiri amagwiritsidwa ntchito ngati gawo laling'ono pamayesero a ma cell a biology kuti aphunzire za jini.Jini la lacZ, lomwe limayika β-galactosidase, nthawi zambiri limaphatikizidwa ndi machitidwe amitundu ina yosangalatsa.Mafotokozedwe a jini ya lacZ ndi hydrolysis ya phenylgalactoside ndi β-galactosidase akhoza kusonyeza chitsanzo ndi mlingo wa chandamale jini kuphunzira.

Makina owonera: Phenylgalactoside ndi gawo lofunikira pamakina owunikira omwe amagwiritsa ntchito ntchito ya β-galactosidase.Chitsanzo chimodzi chodziwika bwino ndi njira yowunika ya buluu-yoyera, yomwe imagwiritsidwa ntchito kuzindikira maselo ophatikizana kapena osinthika pakuyesa kwa biology ya mamolekyulu.Makoloni omwe atenga bwino DNA yowonjezereka kapena kuyanjananso ndi majini adzafotokozera β-galactosidase, zomwe zimatsogolera ku hydrolysis ya phenylgalactoside ndi kupanga mtundu wa buluu.

Kuyeretsa mapuloteni: Nthawi zina, phenylgalactoside ingagwiritsidwe ntchito ngati ligand kwa affinity chromatography kuyeretsa mapuloteni omwe amamangiriza kapena oyambitsidwa ndi β-galactosidase.Puloteni yosangalatsa ikhoza kukhala ndi tag yogwirizana kapena tagi yophatikizika yokhala ndi β-galactosidase-binding domain.Podutsa mapuloteni osakanikirana ndi gawo limodzi ndi immobilized phenylgalactoside, mapuloteni omwe amafunidwa amatha kusungidwa mwachisawawa ndipo kenako amachotsedwa.