TRIS-Acetate CAS: 6850-28-8 Mtengo wa Wopanga

TRIS-Acetate, ndi buffer yomwe imagwiritsidwa ntchito kwambiri poyesa zamoyo ndi zamankhwala. Ndi kuphatikiza kwa Tris base ndi acetic acid, zomwe zimapangitsa kuti pakhale yankho lokhazikika la pH lomwe limagwiritsidwa ntchito kuwongolera ndikusunga pH yofunikira pa ntchito zosiyanasiyana. TRIS-Acetate ndi yothandiza kwambiri pamaphunziro a DNA ndi RNA, chifukwa imapereka malo oyenera a zochita za enzyme, electrophoresis, ndi gel electrophoresis. Imathandiza kusunga bata ndi umphumphu wa nucleic acids panthawi zosiyanasiyana, monga DNA sequencing, polymerase chain reaction (PCR), ndi agarose gel electrophoresis. Kuphatikiza pa kafukufuku wa nucleic acid, TRIS-Acetate imagwiritsidwanso ntchito poyesa mapuloteni olekanitsidwa ndi kutsukidwa, kuchotsa mapuloteni a membrane, ndi kuyesa kwa maselo. Mphamvu yake yosinthasintha yosungira imapangitsa kuti ikhale chida chofunikira m'magawo ambiri ofufuza asayansi, kuonetsetsa kuti zinthu zili bwino kwambiri pakuyankha kwachilengedwe komanso kusunga bata la ma enzyme ndi mapuloteni.

Oda kapena Pemphani Mtengo

Tsatanetsatane wa Zamalonda

Ma tag a Zamalonda

Kugwiritsa Ntchito ndi Zotsatira zake

Tris-acetate (TRIS-Acetate) ndi buffer yomwe imagwiritsidwa ntchito kwambiri mu kuyesa kwa zamoyo ndi zamankhwala. Imapangidwa ndi kuphatikiza kwa tris(hydroxymethyl)aminomethane (Tris) ndi acetic acid, yomwe imagwira ntchito ngati chowongolera pH komanso chokhazikika. pH ya buffer ya TRIS-Acetate nthawi zambiri imakhala pakati pa 7.4 ndi 8.4.
Chotsatira chachikulu cha TRIS-Acetate ndikusunga pH yokhazikika, yomwe ndi yofunika kwambiri pazochitika zambiri zamoyo ndi zamankhwala. Imagwira ntchito ngati chotetezera pochepetsa kusintha kulikonse kwakukulu mu pH komwe kungachitike chifukwa cha ma acid kapena ma base owonjezera panthawi yoyeserera.
TRIS-Acetate imagwiritsa ntchito njira zosiyanasiyana mu biology ya mamolekyulu, biochemistry, ndi biotechnology:
Electrophoresis ya DNA ndi RNA: TRIS-Acetate imagwiritsidwa ntchito kwambiri ngati chotetezera mu agarose ndi polyacrylamide gel electrophoresis. Imapereka malo okhazikika a pH panthawi yolekanitsa zidutswa za DNA ndi RNA kutengera kukula kwawo.
Kusanthula Mapuloteni: Ma buffer a TRIS-Acetate amagwiritsidwa ntchito pa electrophoresis ya mapuloteni, monga SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis). Amaonetsetsa kuti mapuloteni azikhala olimba komanso olekanitsidwa panthawiyi.
Machitidwe a Enzyme: Ma buffer a TRIS-Acetate amagwiritsidwa ntchito nthawi zambiri poyesa ndi kusanthula ma enzyme. Amapereka pH yabwino kwambiri pazochitika zosiyanasiyana za enzymatic ndipo amathandiza kuti ma enzyme agwire ntchito bwino.
Kukulitsa Maselo ndi Minofu: Ma buffer a TRIS-Acetate amagwiritsidwa ntchito mu malo osungira maselo kuti asunge pH yoyenera kuti maselo akule ndi kufalikira. Zimathandiza kusunga mikhalidwe yofunikira ya thupi kuti maselo akhale ndi moyo wabwino.

Kulongedza Zinthu:

Zina Zowonjezera:

Kapangidwe kake	C6H15NO5
Kuyesa	99%
Maonekedwe	Ufa woyera
Nambala ya CAS	6850-28-8
Kulongedza	Kakang'ono komanso kochuluka
Moyo wa Shelufu	zaka 2
Malo Osungirako	Sungani pamalo ozizira komanso ouma
Chitsimikizo	ISO.