Tris(hydroxymethyl)nitromethane CAS:126-11-4

Zotsatira:

Buffering Capacity: Tris imagwira ntchito ngati njira yochepetsera chitetezo chifukwa chakutha kuvomereza kapena kupereka ma protoni, kukhalabe ndi pH yokhazikika pamayankho.Nthawi zambiri amagwiritsidwa ntchito ngati gawo loyambira muzinthu zosungira kuti akhazikitse pH ya zitsanzo zachilengedwe ndi machitidwe.

Mapulogalamu:

Biology ya Molecular: Tris imagwiritsidwa ntchito kwambiri ngati njira yochepetsera mamolekyulu osiyanasiyana, kuphatikiza DNA ndi RNA kudzipatula, PCR, gel electrophoresis, ndi kuyeretsa mapuloteni.Imathandizira kukhala ndi pH yokhazikika, kulola mikhalidwe yabwino kwambiri yochitira ma enzymatic ndi kuyanjana kwa maselo.

Chikhalidwe Cha Maselo: Tris nthawi zambiri imagwiritsidwa ntchito muzofalitsa zama cell kuti azikhala ndi pH yokhazikika komanso osmotic, kuthandizira kukula kwa maselo athanzi komanso kugwira ntchito bwino.

Protein Chemistry: Tris imagwiritsidwa ntchito poyesa chemistry ya protein, monga kukhazikika kwa mapuloteni, kuyesa kukhazikika kwa mapuloteni, ndi maphunziro omanga ma protein-ligand.Imathandizira kukhala ndi pH yofunikira, kuonetsetsa kuti mapuloteni amapindika moyenera komanso ntchito.

Enzymology: Tris amagwiritsidwa ntchito poyesa ma enzymatic osiyanasiyana kuti akwaniritse pH yofunikira pakuchita ma enzymatic.Zimatsimikizira zotsatira zodalirika komanso zobwereketsa, zomwe zimathandiza kuyeza kolondola kwa enzyme kinetics ndi maphunziro oletsa.

Mayeso a Biochemical: Tris imagwiritsidwa ntchito ngati gawo pamayesero ambiri a biochemical chifukwa cha kubisa kwake.Imasunga pH yosalekeza panthawi ya colorimetric, spectrophotometric, ndi ma enzymatic assay, kupititsa patsogolo kulondola ndi kudalirika kwa zotsatira.