3-HYDROXY-4-(5-NITROPYRIDYLAZO)PROPYLANI CAS:143205-66-7

Ndi kafukufuku wa fulorosenti yomwe ili ndi gulu la nitrobenzoxadiazole (NBD), lomwe limasonyeza mphamvu za fluorescence.Gulu la aldehyde limalola kulembedwa kwapadera ndikuzindikira ma biomolecules monga mapuloteni, nucleic acid, ndi lipids.

NBD-aldehyde nthawi zambiri imagwiritsidwa ntchito ngati utoto wokhazikika pakuyesa kuzindikira ndikuwerengera ma protein kapena peptide conjugation reaction, kusintha kwa protein, ndi protein-mapuloteni.Ikhoza kutchula magulu a amino aulere pamapuloteni kapena ma peptides, kupanga zomangira zokhazikika.Izi zimapangitsa kuti zikhale zothandiza pazogwiritsa ntchito monga immunocytochemistry, immunohistochemistry, ndi Western blotting.

Fluorescence ya NBD-aldehyde imatha kusangalatsidwa ndi kuwala kwa ultraviolet kapena buluu, ndipo imatulutsa fulorosenti yobiriwira.Katunduyu amathandizira kuwona ndi kuchuluka kwa ma biomolecules olembedwa pogwiritsa ntchito ma microscopy a fluorescence kapena njira zowonera.