4-Methylumbelliferyl-beta-D-glucopyranoside CAS: 18997-57-4

Zotsatira za 4-Methylumbelliferyl-beta-D-glucopyranoside (MUG) ndizoyenera kukhala gawo lapansi la enzyme beta-glucosidase.Enzyme iyi imadula mgwirizano wa glucosidic wa MUG, zomwe zimapangitsa kuti 4-Methylumbelliferone (4-MU) itulutsidwe.Kugwiritsidwa ntchito kwa MUG makamaka kumakhudza tizilombo toyambitsa matenda, makamaka kuti azindikire ndi kuzindikira mabakiteriya omwe amapanga beta-glucosidase.MUG amagwiritsidwa ntchito kwambiri pozindikira Escherichia coli (E. coli) m'madzi ndi zakudya.E. coli imakhala ndi enzyme beta-glucosidase, yomwe imatha hydrolyze MUG ndikupanga chizindikiro cha fulorosenti pamaso pa kuwala kwa ultraviolet (UV).Gawo la MUG likakhala ndi hydrolyzed, 4-MU yopangidwa imatulutsa fluorescence ya buluu, zomwe zimathandizira kuzindikira mabakiteriya omwe ali ndi ntchito ya beta-glucosidase.Njirayi imagwiritsidwa ntchito poyang'anira zachilengedwe komanso kuyesa chitetezo cha chakudya, chifukwa imapereka njira yofulumira komanso yodziwika bwino yodziwira kuipitsidwa ndi mabakiteriya.Kuphatikiza pa ntchito yake mu microbiology, MUG ingagwiritsidwenso ntchito pophunzira ntchito ndi kuletsa kwa beta-glucosidase mu kafukufuku wa biochemistry ndi molekyulu ya biology.Fluorescence yake imathandizira kuyeza kwa enzyme kinetics ndipo ingagwiritsidwe ntchito poyang'ana zomwe zingatheke zoletsa kapena zoyambitsa ntchito ya beta-glucosidase.Ponseponse, MUG ndi gulu losunthika lomwe limagwiritsidwa ntchito ponseponse m'magulu a microbiology, biochemistry, ndi biology ya molekyulu kuti azindikire. ntchito ya beta-glucosidase ndikuzindikiritsa mabakiteriya omwe amapanga enzyme iyi.