4-NITROPHENYL-ALPHA-D-MANNOPYRANOSIDE CAS:10357-27-4

Ma enzyme magawo: 4NPM angagwiritsidwe ntchito ngati gawo lapansi la michere yosiyanasiyana, kuphatikiza ma glycosidase ndi ma enzymes ogwirizana.Ma enzymes awa amadula mgwirizano wa glycosidic pakati pa mannose ndi 4NPM, zomwe zimapangitsa kuti gawo la nitrophenyl litulutsidwe.Kukula kwa gawo lapansi la hydrolysis kungayesedwe mowoneka bwino poyang'anira kuyamwa kwa gulu la nitrophenyl lomwe latulutsidwa pautali wosiyanasiyana.Izi zimalola ofufuza kuti awone ntchito ya enzyme ndi kinetics.

Kuyesa kwa kagayidwe kachakudya kagayidwe kachakudya: Pogwiritsa ntchito 4NPM ngati gawo laling'ono, ofufuza amatha kuphunzira momwe ma enzymes omwe amakhudzidwa ndi metabolism ya carbohydrate, monga alpha-mannosidases.Ma enzymes awa amatulutsa hydrolyze glycosidic bond mumagulu okhala ndi mannose, ndipo ntchito yawo imatha kuyesedwa poyang'anira kutuluka kwa nitrophenyl moiety kuchokera ku 4NPM.

Maphunziro a Glycosylation: 4NPM itha kugwiritsidwanso ntchito poyesa kufufuza ma enzyme omwe amakhudzidwa ndi njira za glycosylation.Glycosylation ndi njira yolumikizira mamolekyu a shuga ku mapuloteni kapena mamolekyu ena, ndipo ma enzymes ambiri amakhudzidwa ndi izi.Pogwiritsa ntchito 4NPM ngati gawo lapansi lovomerezeka, ofufuza amatha kuyeza kusamutsidwa kwa shuga ku 4NPM ndi michere yapadera yomwe imakhudzidwa ndi machitidwe a glycosylation.

Kuwunika kwa enzyme inhibitors kapena activators: 4NPM ingagwiritsidwe ntchito poyesa kufufuza kwapamwamba kuti azindikire mankhwala omwe amalepheretsa kapena kuyambitsa ma enzymes enieni.Powunika zotsatira za mankhwala oyesa pa hydrolysis kapena kusinthidwa kwa 4NPM ndi ma enzyme omwe akufuna, ofufuza atha kuzindikira omwe angakhale othandizira kapena ma probes amankhwala othandizira pophunzira momwe ma enzyme amagwira.