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PNPG CAS:3150-24-1 Mtengo Wopanga
PNPG, kapena p-nitrophenyl β-D-glucopyranoside, ndi gawo laling'ono la molekyulu lomwe nthawi zambiri limagwiritsidwa ntchito poyesa biochemical kuyesa ntchito ya michere ya glucosidase.Ndi yopanda utoto komanso yopanda fulorosenti, koma ikapangidwa ndi hydrolysis ndi glucosidase, imasinthidwa kukhala p-nitrophenol, yomwe ili ndi mtundu wachikasu ndipo imawoneka mosavuta pa spectrophotometrically.
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ONPG CAS: 369-07-3 Mtengo Wopanga
O-nitrophenyl-β-D-galactopyranoside (ONPG) ndi gawo lapansi lopangira lomwe limagwiritsidwa ntchito poyesa biochemical ndi mamolekyulu a biology kuyesa ntchito ya enzyme β-galactosidase.Amagwiritsidwa ntchito poyesa kuti azindikire mawonekedwe a jini m'makina a bakiteriya, monga Escherichia coli.ONPG ndi gulu lopanda mtundu lomwe limang'ambika ndi β-galactosidase, zomwe zimapangitsa kuti pakhale kutulutsa kwachikasu, o-nitrophenol.Mtundu wachikasu womwe umapangidwa ukhoza kuyeza spectrophotometrically, kupereka muyeso wosalunjika wa zochita za ma enzyme.Kuyesa kogwiritsa ntchito ONPG nthawi zambiri kumatchedwa ONPG assay ndipo ndi njira yomwe imagwiritsidwa ntchito kwambiri pakufufuza kwa mamolekyulu a biology kuwunika kuchuluka kwa ma jini oyendetsedwa ndi lac. operon m'maselo a bakiteriya.
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Nitrotetrazolium Blue Chloride CAS: 298-83-9
Nitrotetrazolium Blue Chloride (NBT) ndi chizindikiro cha redox chomwe chimagwiritsidwa ntchito poyesa zamoyo ndi zamankhwala.Ndi ufa wotumbululuka wachikasu womwe umasanduka buluu ukachepetsedwa, zomwe zimapangitsa kuti zikhale zothandiza kudziwa kupezeka kwa ma enzymes ndi zochita za metabolism.
NBT imakhudzidwa ndi zonyamulira ma elekitironi ndi ma enzymes monga ma dehydrogenases, omwe amakhudzidwa ndi njira zosiyanasiyana zama cell.NBT ikachepetsedwa ndi ma enzymes awa, imapanga buluu formazan precipitate, kulola kudziwika kapena spectrophotometric.
Reagent iyi imagwiritsidwa ntchito poyesa ngati mayeso a NBT kuchepetsa, komwe amagwiritsidwa ntchito poyesa kupuma kwapang'onopang'ono kwa maselo a chitetezo chamthupi.Itha kugwiritsidwanso ntchito powerengera zochitika za ma enzyme ndi njira za metabolic pakufufuza kokhudzana ndi kupsinjika kwa okosijeni, kuthekera kwa ma cell, komanso kusiyanitsa kwa ma cell.
NBT yapeza ntchito m'magawo osiyanasiyana, kuphatikiza microbiology, immunology, ndi cell biology.Ndiwokhazikika, yokhazikika, komanso yosavuta kugwiritsa ntchito, zomwe zimapangitsa kukhala chisankho chodziwika bwino pama protocol ambiri oyesera.
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Neocuproine CAS: 484-11-7 Mtengo Wopanga
Neocuproine ndi chelating wothandizira omwe amagwiritsidwa ntchito kwambiri pazinthu zosiyanasiyana, kuphatikiza ma analytical chemistry ndi pharmaceuticals.Imakhala ndi mgwirizano wamphamvu wa ayoni amkuwa ndipo imapanga ma complexes okhazikika nawo.Katunduyu amapangitsa kuti neocuproine ikhale yothandiza pozindikira komanso kuwerengera mkuwa mu mayankho kapena zitsanzo.Kuphatikiza apo, neocuproine yaphunziridwa chifukwa cha ntchito zake zochizira, makamaka pochiza khansa ndi matenda a neurodegenerative.
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IPTG CAS:367-93-1 Mtengo Wopanga
Isopropyl β-D-1-thiogalactopyranoside (IPTG) ndi analogue yopangidwa ndi lactose yomwe imagwiritsidwa ntchito kwambiri pofufuza kafukufuku wa mamolekyulu a biology ndi biotechnology application.IPTG imagwiritsidwa ntchito kulimbikitsa kufotokozera kwa majini m'makina a bakiteriya, komwe kumakhala ngati choyambitsa mamolekyulu kuti ayambitse kusindikiza kwa jini.
Mukawonjezeredwa ku sing'anga ya kukula, IPTG imatengedwa ndi mabakiteriya ndipo imatha kumangirira ku mapuloteni a lac repressor, kuti asatseke ntchito ya lac operon.Lac operon ndi gulu la majini omwe amakhudzidwa ndi lactose metabolism, ndipo mapuloteni opondereza akachotsedwa, majini amawonekera.
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HATU CAS:148893-10-1 Mtengo Wopanga
HATU (1- [bis(dimethylamino) methylene] -1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxid hexafluorophosphate) ndizomwe zimagwiritsidwa ntchito polumikizira peptide synthesis ndi organic chemistry.
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D-fucose CAS: 3615-37-0 Mtengo Wopanga
D-fucose ndi monosaccharide, makamaka shuga wa carbon 6, womwe uli m'gulu la mashuga osavuta otchedwa hexoses.Ndi isomer ya glucose, yosiyana ndi kasinthidwe ka gulu limodzi la hydroxyl.
D-fucose imapezeka mwachilengedwe m'zamoyo zosiyanasiyana, kuphatikizapo mabakiteriya, bowa, zomera, ndi zinyama.Imagwira ntchito yofunika kwambiri pazachilengedwe zingapo, monga kusanja ma cell, kumatira kwa cell, ndi kaphatikizidwe ka glycoprotein.Ndi gawo la glycolipids, glycoproteins, ndi proteoglycans, zomwe zimakhudzidwa ndi kulumikizana ndi ma cell ndi kuzindikira.
Mwa anthu, D-fucose imakhudzidwanso ndi biosynthesis yamagulu ofunikira a glycan, monga ma antigen a Lewis ndi ma antigen a gulu la magazi, omwe amakhudzanso kuphatikizika kwa magazi komanso kutengeka ndi matenda.
D-fucose ikhoza kupezeka kuchokera kuzinthu zosiyanasiyana, kuphatikizapo udzu wa m'nyanja, zomera, ndi kuwira kwa tizilombo toyambitsa matenda.Amagwiritsidwa ntchito pofufuza ndi zamankhwala, komanso kupanga mankhwala enaake ndi mankhwala opangira mankhwala.
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DDT CAS: 3483-12-3 Mtengo Wopanga
DL-Dithiothreitol, yomwe imadziwikanso kuti DTT, ndi chochepetsera chomwe chimagwiritsidwa ntchito pofufuza za biochemical ndi ma cell biology.Ndi kamolekyu yaing'ono yokhala ndi gulu la thiol (lokhala ndi sulfure) kumapeto kulikonse.
DTT imagwiritsidwa ntchito mobwerezabwereza kuswa zomangira za disulfide m'mapuloteni, zomwe zimathandizira kuwulutsa kapena kuzimitsa.Kuchepetsa kwa zomangira za disulfide ndikofunikira pamachitidwe osiyanasiyana a labotale monga kuyeretsa mapuloteni, gel electrophoresis, ndi maphunziro a protein.DTT itha kugwiritsidwanso ntchito kuteteza magulu a thiol ndikuletsa oxidation panthawi yoyesera.
DTT nthawi zambiri imawonjezeredwa ku mayankho oyeserera pang'onopang'ono, ndipo ntchito yake imadalira kukhalapo kwa mpweya.Ndikofunika kusamalira DTT mosamala chifukwa imakhudzidwa ndi mpweya, kutentha, ndi chinyezi, zomwe zingachepetse mphamvu zake.
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D-(+)-Galactose CAS:59-23-4 Mtengo Wopanga
D-(+) -Galactose ndi shuga wa monosaccharide komanso gawo lofunikira pazachilengedwe zambiri.Ndi shuga wachilengedwe wopezeka muzakudya zambiri, monga zipatso, mkaka, ndi ndiwo zamasamba.
Galactose nthawi zambiri imapangidwa m'thupi kudzera muzochita zingapo za enzymatic.Zimagwira ntchito yofunikira pakulumikizana kwa ma cell, kupanga mphamvu, komanso kuphatikizika kwa mamolekyu ofunikira monga glycolipids, glycoproteins, ndi lactose.
Potengera momwe amagwiritsidwira ntchito, D-(+) -Galactose imagwiritsidwa ntchito kwambiri mu microbiology ndi biotechnology ngati gwero la kaboni mu media media pakukula kwa tizilombo tosiyanasiyana.Amagwiritsidwanso ntchito popanga mankhwala osiyanasiyana a bioactive, mankhwala, ndi zakudya.Kuonjezera apo, amagwiritsidwa ntchito nthawi zambiri ngati chithandizo chachipatala, makamaka poyesa kuyesa ntchito ya chiwindi ndi kuzindikira matenda okhudzana ndi galactose metabolism.
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beta-D-Galactose pentaacetate CAS: 4163-60-4
Beta-D-Galactose pentaacetate ndi mankhwala omwe amachokera ku galactose, shuga wa monosaccharide.Amapangidwa ndi acetylating gulu lililonse la hydroxyl la molekyulu ya galactose yokhala ndi magulu asanu a acetyl.
Pawiri iyi nthawi zambiri imagwiritsidwa ntchito ngati chitetezo cha galactose muzochita zosiyanasiyana zamakina ndi njira zopangira.Mawonekedwe a pentaacetate amathandizira kukhazikika kwa galactose ndikuletsa zochitika zosafunikira kapena kusintha pakachitika.
Kuphatikiza apo, mankhwalawa amatha kugwiritsidwa ntchito ngati kalambulabwalo wa kaphatikizidwe kazinthu zina za galactose.Magulu a acetyl amatha kuchotsedwa mwachisawawa kuti apeze zotumphukira zosiyanasiyana za galactose ndi magulu apadera ogwira ntchito.
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5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium mchere CAS: 129541-41-9
5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide mchere wa sodium ndi mankhwala omwe amagwiritsidwa ntchito kwambiri pofufuza kafukufuku wa labotale ndi matenda.Nthawi zambiri amatchedwa X-Gluc ndipo amagwiritsidwa ntchito kwambiri ngati gawo lapansi pozindikira zochitika za enzyme ya beta-glucuronidase.
Beta-glucuronidase ikapezeka, imadula chomangira cha glucuronide mu X-Gluc, zomwe zimapangitsa kumasulidwa kwa utoto wabuluu wotchedwa 5-bromo-4-chloro-3-indolyl.Izi nthawi zambiri zimagwiritsidwa ntchito pozindikira kapena kuwona mawonekedwe a enzyme ya beta-glucuronidase m'maselo kapena minofu.
Mtundu wamchere wa sodium wa X-Gluc umapangitsa kusungunuka kwake m'madzi amadzimadzi, kumathandizira kugwiritsidwa ntchito kwake pakuyesa ma labotale.X-Gluc imagwiritsidwa ntchito makamaka pakufufuza kwa mamolekyulu a biology kuti aphunzire za jini, zochitika zolimbikitsa, komanso kuyesa kwa majini atolankhani.Itha kugwiritsidwanso ntchito kuzindikira kukhalapo kwa zamoyo zomwe zimapanga beta-glucuronidase, monga mabakiteriya ena, m'maphunziro a microbiological.
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4-Nitrophenyl-beta-D-xylopyranoside CAS:2001-96-9
4-Nitrophenyl-beta-D-xylopyranoside ndi gawo laling'ono la chromogenic lomwe limagwiritsidwa ntchito poyesa ma enzymatic kuti azindikire ndikuyesa ntchito ya michere yotchedwa beta-xylosidases.
