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1,2,3,4,6-Penta-O-acetyl-D-mannopyranose CAS:25941-03-1
1,2,3,4,6-Penta-O-acetyl-D-mannopyranose ndi mankhwala omwe amachokera ku D-mannose, shuga wosavuta.Ndizochokera komwe magulu a acetyl amalumikizidwa kumagulu asanu mwa asanu ndi limodzi a hydroxyl omwe amapezeka mu molekyulu ya mannose.Mtundu wa acetylated wa D-mannose umagwiritsidwa ntchito popanga organic synthesis ndi kafukufuku wamankhwala ngati chomangira kapena zoyambira zopangira mamolekyu ovuta kwambiri.Magulu a acetyl amapereka bata ndipo amatha kusintha reactivity ndi katundu wa pawiri.
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1,2,3,4-Di-O-Isopropylidene-alpha-D-galactopyranose CAS:4064-06-6
1,2:3,4-Di-O-isopropylidene-D-galactopyranose ndi mankhwala omwe ali m'banja la galactopyranose derivatives.Amagwiritsidwa ntchito kwambiri mu organic chemistry ngati woteteza magulu a hydroxyl omwe amapezeka mu shuga, makamaka galactose.Pawiriyi amapangidwa pochita D-galactose ndi acetone kupanga chotengera cha diacetone, chomwe chimapangidwa ndi asidi kupanga chotengera cha di-O-isopropylidene.Izi zotumphukira zimateteza magulu a hydroxyl, kuteteza zosafunika panthawi ya kaphatikizidwe ka mankhwala, ndipo zitha kuchotsedwa mwachisawawa kuti apangenso gawo loyambirira.Kapangidwe kake kakang'ono komanso kukhazikika kwake kumapangitsa kuti ikhale yopindulitsa pazinthu zosiyanasiyana m'munda wa organic synthesis.
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X-GAL CAS:7240-90-6 Mtengo Wopanga
5-Bromo-4-chloro-3-indolyl-β-D-galactoside (X-Gal) ndi gawo lodziwika bwino la chromogenic lomwe limagwiritsidwa ntchito mu biology ndi biochemistry.Amagwiritsidwa ntchito kwambiri pozindikira jini ya lacZ, yomwe imayika enzyme β-galactosidase.
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PNPG CAS:3150-24-1 Mtengo Wopanga
PNPG, kapena p-nitrophenyl β-D-glucopyranoside, ndi gawo laling'ono la molekyulu lomwe nthawi zambiri limagwiritsidwa ntchito poyesa biochemical kuyesa ntchito ya michere ya glucosidase.Ndi yopanda utoto komanso yopanda fulorosenti, koma ikapangidwa ndi hydrolysis ndi glucosidase, imasinthidwa kukhala p-nitrophenol, yomwe ili ndi mtundu wachikasu ndipo imawoneka mosavuta pa spectrophotometrically.
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ONPG CAS: 369-07-3 Mtengo Wopanga
O-nitrophenyl-β-D-galactopyranoside (ONPG) ndi gawo lapansi lopangira lomwe limagwiritsidwa ntchito poyesa biochemical ndi mamolekyulu a biology kuyesa ntchito ya enzyme β-galactosidase.Amagwiritsidwa ntchito poyesa kuti azindikire mawonekedwe a jini m'makina a bakiteriya, monga Escherichia coli.ONPG ndi gulu lopanda mtundu lomwe limang'ambika ndi β-galactosidase, zomwe zimapangitsa kuti pakhale kutulutsa kwachikasu, o-nitrophenol.Mtundu wachikasu womwe umapangidwa ukhoza kuyeza spectrophotometrically, kupereka muyeso wosalunjika wa zochita za ma enzyme.Kuyesa kogwiritsa ntchito ONPG nthawi zambiri kumatchedwa ONPG assay ndipo ndi njira yomwe imagwiritsidwa ntchito kwambiri pakufufuza kwa mamolekyulu a biology kuwunika kuchuluka kwa ma jini oyendetsedwa ndi lac. operon m'maselo a bakiteriya.
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Nitrotetrazolium Blue Chloride CAS: 298-83-9
Nitrotetrazolium Blue Chloride (NBT) ndi chizindikiro cha redox chomwe chimagwiritsidwa ntchito poyesa zamoyo ndi zamankhwala.Ndi ufa wotumbululuka wachikasu womwe umasanduka buluu ukachepetsedwa, zomwe zimapangitsa kuti zikhale zothandiza kudziwa kupezeka kwa ma enzymes ndi zochita za metabolism.
NBT imakhudzidwa ndi zonyamulira ma elekitironi ndi ma enzymes monga ma dehydrogenases, omwe amakhudzidwa ndi njira zosiyanasiyana zama cell.NBT ikachepetsedwa ndi ma enzymes awa, imapanga buluu formazan precipitate, kulola kudziwika kapena spectrophotometric.
Reagent iyi imagwiritsidwa ntchito poyesa ngati mayeso a NBT kuchepetsa, komwe amagwiritsidwa ntchito poyesa kupuma kwapang'onopang'ono kwa maselo a chitetezo chamthupi.Itha kugwiritsidwanso ntchito powerengera zochitika za ma enzyme ndi njira za metabolic pakufufuza kokhudzana ndi kupsinjika kwa okosijeni, kuthekera kwa ma cell, komanso kusiyanitsa kwa ma cell.
NBT yapeza ntchito m'magawo osiyanasiyana, kuphatikiza microbiology, immunology, ndi cell biology.Ndiwokhazikika, yokhazikika, komanso yosavuta kugwiritsa ntchito, zomwe zimapangitsa kukhala chisankho chodziwika bwino pama protocol ambiri oyesera.
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Neocuproine CAS: 484-11-7 Mtengo Wopanga
Neocuproine ndi chelating wothandizira omwe amagwiritsidwa ntchito kwambiri pazinthu zosiyanasiyana, kuphatikiza ma analytical chemistry ndi pharmaceuticals.Imakhala ndi mgwirizano wamphamvu wa ayoni amkuwa ndipo imapanga ma complexes okhazikika nawo.Katunduyu amapangitsa kuti neocuproine ikhale yothandiza pozindikira komanso kuwerengera mkuwa mu mayankho kapena zitsanzo.Kuphatikiza apo, neocuproine yaphunziridwa chifukwa cha ntchito zake zochizira, makamaka pochiza khansa ndi matenda a neurodegenerative.
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IPTG CAS:367-93-1 Mtengo Wopanga
Isopropyl β-D-1-thiogalactopyranoside (IPTG) ndi analogue yopangidwa ndi lactose yomwe imagwiritsidwa ntchito kwambiri pofufuza kafukufuku wa mamolekyulu a biology ndi biotechnology application.IPTG imagwiritsidwa ntchito kulimbikitsa kufotokozera kwa majini m'makina a bakiteriya, komwe kumakhala ngati choyambitsa mamolekyulu kuti ayambitse kusindikiza kwa jini.
Mukawonjezeredwa ku sing'anga ya kukula, IPTG imatengedwa ndi mabakiteriya ndipo imatha kumangirira ku mapuloteni a lac repressor, kuti asatseke ntchito ya lac operon.Lac operon ndi gulu la majini omwe amakhudzidwa ndi lactose metabolism, ndipo mapuloteni opondereza akachotsedwa, majini amawonekera.
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HATU CAS:148893-10-1 Mtengo Wopanga
HATU (1- [bis(dimethylamino) methylene] -1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxid hexafluorophosphate) ndizomwe zimagwiritsidwa ntchito polumikizira peptide synthesis ndi organic chemistry.
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D-fucose CAS: 3615-37-0 Mtengo Wopanga
D-fucose ndi monosaccharide, makamaka shuga wa carbon 6, womwe uli m'gulu la mashuga osavuta otchedwa hexoses.Ndi isomer ya glucose, yosiyana ndi kasinthidwe ka gulu limodzi la hydroxyl.
D-fucose imapezeka mwachilengedwe m'zamoyo zosiyanasiyana, kuphatikizapo mabakiteriya, bowa, zomera, ndi zinyama.Imagwira ntchito yofunika kwambiri pazachilengedwe zingapo, monga kusanja ma cell, kumatira kwa cell, ndi kaphatikizidwe ka glycoprotein.Ndi gawo la glycolipids, glycoproteins, ndi proteoglycans, zomwe zimakhudzidwa ndi kulumikizana ndi ma cell ndi kuzindikira.
Mwa anthu, D-fucose imakhudzidwanso ndi biosynthesis yamagulu ofunikira a glycan, monga ma antigen a Lewis ndi ma antigen a gulu la magazi, omwe amakhudzanso kuphatikizika kwa magazi komanso kutengeka ndi matenda.
D-fucose ikhoza kupezeka kuchokera kuzinthu zosiyanasiyana, kuphatikizapo udzu wa m'nyanja, zomera, ndi kuwira kwa tizilombo toyambitsa matenda.Amagwiritsidwa ntchito pofufuza ndi zamankhwala, komanso kupanga mankhwala enaake ndi mankhwala opangira mankhwala.
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DDT CAS: 3483-12-3 Mtengo Wopanga
DL-Dithiothreitol, yomwe imadziwikanso kuti DTT, ndi chochepetsera chomwe chimagwiritsidwa ntchito pofufuza za biochemical ndi ma cell biology.Ndi kamolekyu yaing'ono yokhala ndi gulu la thiol (lokhala ndi sulfure) kumapeto kulikonse.
DTT imagwiritsidwa ntchito mobwerezabwereza kuswa zomangira za disulfide m'mapuloteni, zomwe zimathandizira kuwulutsa kapena kuzimitsa.Kuchepetsa kwa zomangira za disulfide ndikofunikira pamachitidwe osiyanasiyana a labotale monga kuyeretsa mapuloteni, gel electrophoresis, ndi maphunziro a protein.DTT itha kugwiritsidwanso ntchito kuteteza magulu a thiol ndikuletsa oxidation panthawi yoyesera.
DTT nthawi zambiri imawonjezeredwa ku mayankho oyeserera pang'onopang'ono, ndipo ntchito yake imadalira kukhalapo kwa mpweya.Ndikofunika kusamalira DTT mosamala chifukwa imakhudzidwa ndi mpweya, kutentha, ndi chinyezi, zomwe zingachepetse mphamvu zake.
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D-(+)-Galactose CAS:59-23-4 Mtengo Wopanga
D-(+) -Galactose ndi shuga wa monosaccharide komanso gawo lofunikira pazachilengedwe zambiri.Ndi shuga wachilengedwe wopezeka muzakudya zambiri, monga zipatso, mkaka, ndi ndiwo zamasamba.
Galactose nthawi zambiri imapangidwa m'thupi kudzera muzochita zingapo za enzymatic.Zimagwira ntchito yofunikira pakulumikizana kwa ma cell, kupanga mphamvu, komanso kuphatikizika kwa mamolekyu ofunikira monga glycolipids, glycoproteins, ndi lactose.
Potengera momwe amagwiritsidwira ntchito, D-(+) -Galactose imagwiritsidwa ntchito kwambiri mu microbiology ndi biotechnology ngati gwero la kaboni mu media media pakukula kwa tizilombo tosiyanasiyana.Amagwiritsidwanso ntchito popanga mankhwala osiyanasiyana a bioactive, mankhwala, ndi zakudya.Kuonjezera apo, amagwiritsidwa ntchito nthawi zambiri ngati chithandizo chachipatala, makamaka poyesa kuyesa ntchito ya chiwindi ndi kuzindikira matenda okhudzana ndi galactose metabolism.
