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Tapso Sodium CAS: 105140-25-8 Mtengo wa Wopanga
3-[N-Tris(hydroxymethyl)methylamino]-2-hydroxypropanesulfonic acid,Inesa zwitterionic buffering agent mu mawonekedwe a sodium salt. Amagwiritsidwa ntchito kwambiri muzoyesera za biochemical ndi molecular biology kuti asunge pH yokhazikika mu mayankho.It Imasungunuka kwambiri m'madzi ndipo ili ndi pKa yofanana ndi pH ya thupi, zomwe zimapangitsa kuti ikhale yoyenera kugwiritsidwa ntchito m'njira zosiyanasiyana monga kuyeretsa mapuloteni, kuyesa ma enzyme, kupanga maselo, ndi electrophoresis. Kapangidwe kake ka mankhwala komanso kugwirizana kwake ndi machitidwe a zamoyo zimapangitsa kuti ikhale gawo lofunikira kwambiri m'mapuloteni osiyanasiyana a labotale.
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4-Nitrophenyl phosphate di(tris) mchere CAS:68189-42-4
Mchere wa 4-Nitrophenyl phosphate di(tris) ndi mankhwala omwe amagwiritsidwa ntchito pofufuza ndi kuzindikira matenda a biochemical. Umagwiritsidwa ntchito kwambiri ngati gawo la mayeso a alkaline phosphatase enzyme. Mcherewu, ukaphikidwa ndi alkaline phosphatase, umapanga chinthu chachikasu chomwe chingayesedwe mosavuta ndi spectrophotometric. Izi zimagwiritsidwa ntchito kwambiri m'njira zosiyanasiyana, kuphatikizapo mayeso a enzyme-linked immunosorbent assays (ELISAs) ndi kuzindikira zochitika za phosphatase. Mphamvu ya mtundu wachikasu imagwirizana mwachindunji ndi ntchito ya enzyme, zomwe zimathandiza kuti kuchuluka kwa alkaline phosphatase kuwerengedwe molondola m'zitsanzo zamoyo. Mchere wa 4-Nitrophenyl phosphate di(tris) ndi wokhazikika, wosungunuka m'madzi, komanso wovuta kwambiri, zomwe zimapangitsa kuti ukhale chisankho chodziwika bwino cha mayeso a enzyme mu kafukufuku wa zamoyo ndi zamankhwala.
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Nitrotetrazolium blue chloride CAS:298-83-9
Nitrotetrazolium blue chloride, yomwe imadziwikanso kuti NBT kapena NBT-Cl, ndi mankhwala omwe amagwiritsidwa ntchito kwambiri poyesa zamoyo ndi zamankhwala. Ndi ufa wachikasu wopepuka womwe umasungunuka m'madzi.
NBT imagwiritsidwa ntchito nthawi zambiri ngati chizindikiro chodziwira kupezeka kwa ma superoxide radicals kapena ma enzyme omwe amawapanga. Imasintha mtundu ikachepetsedwa ndi ma superoxide radicals, kusintha kuchoka pachikasu kupita ku formazan precipitate yabuluu. Izi nthawi zambiri zimagwiritsidwa ntchito poyesa kuphunzira za kupsinjika kwa okosijeni, kupuma kwa maselo, ndi ntchito ya ma enzyme.
NBT imagwiritsidwa ntchito kwambiri m'njira zosiyanasiyana, monga kuchepetsa nitroblue tetrazolium, kuti iwonetse kupanga kwa superoxide m'maselo ndi minofu. Yagwiritsidwanso ntchito mu immunohistochemistry ndi histochemistry kuti ipeze ndikuzindikira ma enzyme ena ndi zigawo zina za maselo.
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NSP-AS CAS:211106-69-3 Mtengo wa Wopanga
Mchere wamkati wa 3-[9-(((3-(carboxypropyl)[4-Methxylphenyl]sulfonyl)aMine)carboxyl]-10-acridiniuMyl)-1-propanesulfonate ndi molekyulu yovuta yokhala ndi gawo la acridinium lolumikizidwa ku mchere wamkati wa propanesulfonate. Uli ndi gulu la carboxypropyl, gulu la 4-Methxylphenylsulfonyl, ndi gulu la amine. Mchere uwu mwina umapangidwa kuti ugwiritsidwe ntchito m'malo ena monga chemistry, mankhwala, kapena kafukufuku.
Kuphatikiza apo, NAC yawonetsa lonjezo lothandiza thanzi la chiwindi mwa kuthandiza kuchotsa zinthu zoopsa, monga acetaminophen, mankhwala ochepetsa ululu. Ikhozanso kuteteza chiwindi ku kuwonongeka komwe kumachitika chifukwa cha kumwa mowa.
Kuwonjezera pa mphamvu zake zoteteza ku ma antioxidants ndi kupuma, NAC yafufuzidwa kuti ipeze ubwino wake pa thanzi la maganizo. Kafukufuku wina akusonyeza kuti ikhoza kukhala ndi zotsatira zabwino pa matenda a maganizo, monga kuvutika maganizo ndi matenda okakamiza (OCD).
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L-Leucine p-nitroanilide hydrochloride CAS:16010-98-3
L-Leucine p-nitroanilide hydrochloride ndi mankhwala omwe ali m'gulu la zinthu zochokera ku p-nitroanilide. Ndi ufa wa kristalo woyera mpaka woyera womwe umagwiritsidwa ntchito kwambiri pofufuza za biochemical ndi kuyesa ma enzyme.
L-Leucine p-nitroanilide hydrochloride nthawi zambiri imagwiritsidwa ntchito ngati gawo loyezera ntchito ya ma enzyme monga ma protease ndi ma peptidases. Pamene ma enzyme amenewa amagawanika L-Leucine p-nitroanilide hydrochloride, imatulutsa p-nitroaniline, yomwe imatha kuzindikirika ndi spectrophotometric. Kuchuluka kwa kusintha kwa mtundu kumafanana mwachindunji ndi ntchito ya enzymatic, zomwe zimapangitsa kuti kuchuluka kwa ntchito ya enzyme kuwerengedwe m'zitsanzo zamoyo.
Mankhwalawa amasungunuka m'madzi ndipo amatha kusungunuka mosavuta m'njira zoyenera zogwiritsira ntchito poyesa ma enzyme. Ndikofunikira kusunga L-Leucine p-nitroanilide hydrochloride pamalo ozizira komanso ouma kuti ikhale yolimba.
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Guanidine Thiocyanate Sulfate CAS:593-84-0
Guanidine thiocyanate sulfate ndi mankhwala ochokera ku ma ayoni a guanidine, thiocyanate, ndi sulfate. Amagwiritsidwa ntchito kwambiri mu kafukufuku wa sayansi ya zamoyo ndi biochemistry ngati chaotropic.
Guanidine thiocyanate sulfate nthawi zambiri imagwiritsidwa ntchito mu njira zolekanitsira DNA ndi RNA kuti isokoneze ma cell membranes ndi mapuloteni owonongeka, zomwe zimathandiza kuti ma nucleic acids atulutsidwe bwino. Zimathandiza kuti DNA ndi RNA zisungunuke, zomwe zimathandiza kuti zilekanitsidwe ndi kuyeretsedwa kuchokera ku zitsanzo zosiyanasiyana zamoyo.
Chifukwa cha mphamvu zake za chaotropic, guanidine thiocyanate sulfate imathandiza kuswa mgwirizano wa hydrogen ndi kuyanjana kwina kosagwirizana ndi covalent, zomwe zimathandiza kulekanitsa ma nucleic acids ndi mapuloteni, lipids, ndi zigawo zina za ma cell. Zimathandizanso kuletsa ntchito ya RNase, kuonetsetsa kuti ma nucleic acids otulutsidwa ndi oyera komanso olondola..
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BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine mchere CAS:6578-06-9
BCIP-Toluidine, yomwe imadziwikanso kuti mchere wa 5-Bromo-4-chloro-3-indolylphosphate-p-toluidine, ndi mankhwala omwe amagwiritsidwa ntchito kwambiri mu kafukufuku wa zamoyo zama molekyulu ndi biochemistry. Amagwiritsidwa ntchito makamaka ngati gawo la kafukufuku wa enzyme-linked immunosorbent assays (ELISAs) kapena njira zoyeretsera za immunohistochemical kuti azindikire ntchito ya alkaline phosphatase.
Pamene BCIP-Toluidine yadulidwa ndi alkaline phosphatase, imadutsa mu kusintha kwa mtundu, zomwe zimapangitsa kuti pakhale buluu-wofiirira. Izi zimathandiza kuti pakhale kuwonetsa ndi kuwerengera ntchito ya alkaline phosphatase m'zitsanzo. Mphamvu ya mtunduwo imatha kuyezedwa pogwiritsa ntchito spectrophotometric ndipo imagwirizana mwachindunji ndi ntchito ya enzyme.
Kugwiritsa ntchito BCIP-Toluidine ngati substrate kumachitika makamaka pakugwiritsa ntchito ma label a DNA kapena RNA, monga in situ hybridization (ISH) kapena Southern and Northern blotting. Kumapereka njira yodalirika komanso yodziwira bwino kupezeka kwa ma nucleic acid omwe ali mu zitsanzo.
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3-Aminophthalhydrazide CAS:521-31-3
3-Aminophthalhydrazide ndi mankhwala omwe ali ndi formula ya molekyulu C8H8N2O. Amatchedwa kuti aminohydrazide derivative ya phthalic acid. Mankhwalawa amapangidwa ndi msana wa phthalic acid wokhala ndi maatomu awiri a hydrogen omwe amalowedwa m'malo ndi gulu la amino ndi gulu la hydrazine.
3-Aminophthalhydrazide imagwira ntchito zosiyanasiyana m'munda wa mankhwala ndi mankhwala achilengedwe. Ingagwiritsidwe ntchito ngati chinthu choyambira popanga mankhwala osiyanasiyana ndi utoto. Kuphatikiza apo, nthawi zambiri imagwiritsidwa ntchito pokonzekera maziko a Schiff, omwe ndi ofunikira kwambiri pakupanga kwachilengedwe.
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4-Aminoantipyrine CAS: 83-07-8
4-Aminoantipyrine, yomwe imadziwikanso kuti aminoantipyrine kapena aminopyrine, ndi mankhwala opangidwa ndi zinthu zachilengedwe. Amagwiritsidwa ntchito kwambiri ngati reagent mu chemistry yosanthula ndi makampani opanga mankhwala. Kapangidwe kake ka mankhwala kamakhala ndi mphete ya aromatic yokhala ndi gulu la amino (NH2) ndi gulu la pyrazolone, zomwe zimapangitsa kuti ikhale mankhwala ogwiritsidwa ntchito m'njira zosiyanasiyana.
Mu chemistry yowunikira, 4-Aminoantipyrine imagwiritsidwa ntchito kwambiri ngati chothandizira cha chromogenic mu mayeso a colorimetric. Imayankhidwa ndi zinthu zina, monga phenols ndi peroxides, kuti ipange mitundu yosiyanasiyana yomwe imatha kuyezedwa ndi spectrophotometric. Kapangidwe kameneka kamaipangitsa kukhala yothandiza mu mayeso osiyanasiyana, kuphatikizapo kudziwa ma enzyme monga peroxidase, glucose, ndi amylase.
Mu makampani opanga mankhwala, 4-Aminoantipyrine yakhala ikugwiritsidwa ntchito kale ngati mankhwala ochepetsa ululu komanso oletsa kupweteka kwa minofu. .
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TAPS-NA CAS:91000-53-2 Mtengo wa Wopanga
N-[Tris(hydroxymethyl)methyl]-3-aminopropanesulfonic acid sodium salt, yomwe imadziwikanso kuti TAPS-Na, ndi mankhwala omwe amagwiritsidwa ntchito kwambiri ngati chotetezera m'njira zosiyanasiyana zamoyo ndi zamankhwala. Ndi mankhwala a zwitterionic, zomwe zikutanthauza kuti ali ndi mphamvu zabwino komanso zoyipa, zomwe zimathandiza kusunga pH yokhazikika mu mayankho.
TAPS-Na ili ndi pH ya pafupifupi 7.7 mpaka 9.1, zomwe zimapangitsa kuti ikhale yoyenera kugwiritsidwa ntchito pofunikira pH yokhala ndi alkaline pang'ono. Nthawi zambiri imagwiritsidwa ntchito pofufuza mapuloteni ndi ma enzyme, komanso mu njira za biology monga gel electrophoresis ndi DNA sequencing.
Monga mchere wa sodium, TAPS-Na imasungunuka kwambiri m'madzi, zomwe zimapangitsa kuti zikhale zosavuta kukonzekera njira zosungiramo zinthu. Imadziwika kuti ndi yopanda poizoni komanso yocheperako kusokoneza mamolekyulu a zamoyo, zomwe zimapangitsa kuti ikhale chisankho chabwino kwambiri pakuyesera kambiri.
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5,5-Dithiobis (2-nitrobenzoic acid) CAS:69-78-3
5,5-Dithiobis (2-nitrobenzoic acid), yomwe imadziwikanso kuti DTNB kapena Ellman's reagent, ndi mankhwala omwe amagwiritsidwa ntchito kwambiri pofufuza zamankhwala ndi zamankhwala. Ndi ufa wachikasu, wa kristalo womwe umasungunuka kwambiri m'madzi ndi zinthu zachilengedwe.
DTNB imagwiritsidwa ntchito makamaka poyesa kuchuluka kwa magulu a sulfhydryl (-SH) m'mapuloteni ndi ma peptide. Chosakanizacho chimagwira ntchito ndi ma thiol, zomwe zimapangitsa kuti pakhale disulfide yosakanikirana ndikutulutsa 2-nitro-5-thiobenzoate, yomwe ili ndi mtundu wachikasu womwe ungayesedwe ndi spectrophotometric. Poyesa kuchuluka kwa mtundu wachikasu, n'zotheka kuyerekeza kuchuluka kwa ma thiols mu chitsanzo. Njirayi nthawi zambiri imagwiritsidwa ntchito pophunzira momwe mapuloteni amagwirira ntchito, kupindika kwa mapuloteni, ndi machitidwe a enzymatic omwe amakhudza magulu a thiol.
Kuwonjezera pa kuwerengera kwa thiol, DTNB yagwiritsidwa ntchito m'magwiritsidwe ena, monga kuwunika momwe maselo alili, kuphunzira kukhazikika kwa mankhwala achitsulo-sulfure, komanso kuyeza ntchito ya ma enzyme osiyanasiyana.
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PHENOLPHTHALEIN MONOPHOSPHATE BIS(CYCLOHEXYLAMMONIUM) Mchere CAS:14815-59-9
Mchere wa dicyclohexylammonium ndi mankhwala omwe amachokera ku phenolphthalein, chizindikiro cha pH chomwe chimagwiritsidwa ntchito kwambiri m'malo ochitira kafukufuku. Mchere wa dicyclohexylammonium wa phenolphthalein monophosphate nthawi zambiri umagwiritsidwa ntchito ngati chothandizira pakufufuza za biochemical ndi molecular biology. Umagwiritsidwa ntchito makamaka poyesa kapena kuyesa kuti upezeke ndikuyezera kukhalapo kwa ma enzyme ena, kuphatikizapo omwe amagwira ntchito mu ATP (adenosine triphosphate) hydrolysis kapena phosphatase activities. Kapangidwe kake kapadera kamapangitsa kuti ikhale chida chothandiza pakufufuza kwasayansi kokhudzana ndi kinetics ya enzyme ndi njira zama cell..
