Nitrotetrazolium Blue Chloride CAS: 298-83-9

Nitrotetrazolium Blue Chloride (NBT) ndi chizindikiro cha redox chomwe chimagwiritsidwa ntchito poyesa zamoyo ndi zamankhwala.Ndi ufa wotumbululuka wachikasu womwe umasanduka buluu ukachepetsedwa, zomwe zimapangitsa kuti zikhale zothandiza kudziwa kupezeka kwa ma enzymes ndi zochita za metabolism.

Chotsatira chachikulu cha NBT ndi mapangidwe a blue formazan precipitate pamene achepetsedwa ndi ma enzymes ena.Kusintha kwa mtundu uku kumapangitsa kuti pakhale mawonekedwe kapena mawonekedwe a spectrophotometric a enzyme.

NBT ili ndi ntchito zosiyanasiyana pakufufuza ndi kufufuza.Nazi zina mwazogwiritsa ntchito zake zoyambirira:

Kuyesa kwa ma enzyme: NBT ikhoza kugwiritsidwa ntchito kuyeza zochita za dehydrogenases, zomwe zimakhudzidwa ndi njira monga kupuma kwa ma cell ndi metabolism.Poyang'anira kuchepetsedwa kwa NBT kukhala formazan, ofufuza angathe kuyesa ntchito ya ma enzymes.

Kuwunika kwa chitetezo chamthupi: NBT imagwiritsidwa ntchito poyesa kuchepetsa kuchepetsa kwa NBT kuyesa kupuma kwa maselo a chitetezo chamthupi, makamaka phagocytes.Mayesowa amayesa kuthekera kwa maselowa kupanga mitundu ya okosijeni yokhazikika, yomwe imatha kuchepetsa NBT ndikupanga mvula yabuluu.

Kafukufuku wa Microbiology: NBT imagwiritsidwa ntchito mu Microbiology kuphunzira kagayidwe kazachilengedwe ndikuwunika momwe ma enzyme ena amagwirira ntchito.Mwachitsanzo, amagwiritsidwa ntchito pozindikira mabakiteriya nitrate reductase kapena mabakiteriya omwe amapanga formazan.

Maphunziro a magwiridwe antchito a ma cell: Kuchepetsa kwa NBT kumalola ofufuza kuti awone momwe kagayidwe kake kamagwirira ntchito komanso kuthekera kwa maselo.Poyerekeza kukula kwa chinthu chamtundu wa buluu formazan, ndizotheka kudziwa kuchuluka kwa maselo otheka mu zitsanzo zomwe zaperekedwa.