-
BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine mchere CAS:6578-06-9
BCIP-Toluidine, yomwe imadziwikanso kuti mchere wa 5-Bromo-4-chloro-3-indolylphosphate-p-toluidine, ndi mankhwala omwe amagwiritsidwa ntchito kwambiri mu kafukufuku wa zamoyo zama molekyulu ndi biochemistry. Amagwiritsidwa ntchito makamaka ngati gawo la kafukufuku wa enzyme-linked immunosorbent assays (ELISAs) kapena njira zoyeretsera za immunohistochemical kuti azindikire ntchito ya alkaline phosphatase.
Pamene BCIP-Toluidine yadulidwa ndi alkaline phosphatase, imadutsa mu kusintha kwa mtundu, zomwe zimapangitsa kuti pakhale buluu-wofiirira. Izi zimathandiza kuti pakhale kuwonetsa ndi kuwerengera ntchito ya alkaline phosphatase m'zitsanzo. Mphamvu ya mtunduwo imatha kuyezedwa pogwiritsa ntchito spectrophotometric ndipo imagwirizana mwachindunji ndi ntchito ya enzyme.
Kugwiritsa ntchito BCIP-Toluidine ngati substrate kumachitika makamaka pakugwiritsa ntchito ma label a DNA kapena RNA, monga in situ hybridization (ISH) kapena Southern and Northern blotting. Kumapereka njira yodalirika komanso yodziwira bwino kupezeka kwa ma nucleic acid omwe ali mu zitsanzo.
-
3-Aminophthalhydrazide CAS:521-31-3
3-Aminophthalhydrazide ndi mankhwala omwe ali ndi formula ya molekyulu C8H8N2O. Amatchedwa kuti aminohydrazide derivative ya phthalic acid. Mankhwalawa amapangidwa ndi msana wa phthalic acid wokhala ndi maatomu awiri a hydrogen omwe amalowedwa m'malo ndi gulu la amino ndi gulu la hydrazine.
3-Aminophthalhydrazide imagwira ntchito zosiyanasiyana m'munda wa mankhwala ndi mankhwala achilengedwe. Ingagwiritsidwe ntchito ngati chinthu choyambira popanga mankhwala osiyanasiyana ndi utoto. Kuphatikiza apo, nthawi zambiri imagwiritsidwa ntchito pokonzekera maziko a Schiff, omwe ndi ofunikira kwambiri pakupanga kwachilengedwe.
-
4-Aminoantipyrine CAS: 83-07-8
4-Aminoantipyrine, yomwe imadziwikanso kuti aminoantipyrine kapena aminopyrine, ndi mankhwala opangidwa ndi zinthu zachilengedwe. Amagwiritsidwa ntchito kwambiri ngati reagent mu chemistry yosanthula ndi makampani opanga mankhwala. Kapangidwe kake ka mankhwala kamakhala ndi mphete ya aromatic yokhala ndi gulu la amino (NH2) ndi gulu la pyrazolone, zomwe zimapangitsa kuti ikhale mankhwala ogwiritsidwa ntchito m'njira zosiyanasiyana.
Mu chemistry yowunikira, 4-Aminoantipyrine imagwiritsidwa ntchito kwambiri ngati chothandizira cha chromogenic mu mayeso a colorimetric. Imayankhidwa ndi zinthu zina, monga phenols ndi peroxides, kuti ipange mitundu yosiyanasiyana yomwe imatha kuyezedwa ndi spectrophotometric. Kapangidwe kameneka kamaipangitsa kukhala yothandiza mu mayeso osiyanasiyana, kuphatikizapo kudziwa ma enzyme monga peroxidase, glucose, ndi amylase.
Mu makampani opanga mankhwala, 4-Aminoantipyrine yakhala ikugwiritsidwa ntchito kale ngati mankhwala ochepetsa ululu komanso oletsa kupweteka kwa minofu. .
-
TAPS-NA CAS:91000-53-2 Mtengo wa Wopanga
N-[Tris(hydroxymethyl)methyl]-3-aminopropanesulfonic acid sodium salt, yomwe imadziwikanso kuti TAPS-Na, ndi mankhwala omwe amagwiritsidwa ntchito kwambiri ngati chotetezera m'njira zosiyanasiyana zamoyo ndi zamankhwala. Ndi mankhwala a zwitterionic, zomwe zikutanthauza kuti ali ndi mphamvu zabwino komanso zoyipa, zomwe zimathandiza kusunga pH yokhazikika mu mayankho.
TAPS-Na ili ndi pH ya pafupifupi 7.7 mpaka 9.1, zomwe zimapangitsa kuti ikhale yoyenera kugwiritsidwa ntchito pofunikira pH yokhala ndi alkaline pang'ono. Nthawi zambiri imagwiritsidwa ntchito pofufuza mapuloteni ndi ma enzyme, komanso mu njira za biology monga gel electrophoresis ndi DNA sequencing.
Monga mchere wa sodium, TAPS-Na imasungunuka kwambiri m'madzi, zomwe zimapangitsa kuti zikhale zosavuta kukonzekera njira zosungiramo zinthu. Imadziwika kuti ndi yopanda poizoni komanso yocheperako kusokoneza mamolekyulu a zamoyo, zomwe zimapangitsa kuti ikhale chisankho chabwino kwambiri pakuyesera kambiri.
-
5,5-Dithiobis (2-nitrobenzoic acid) CAS:69-78-3
5,5-Dithiobis (2-nitrobenzoic acid), yomwe imadziwikanso kuti DTNB kapena Ellman's reagent, ndi mankhwala omwe amagwiritsidwa ntchito kwambiri pofufuza zamankhwala ndi zamankhwala. Ndi ufa wachikasu, wa kristalo womwe umasungunuka kwambiri m'madzi ndi zinthu zachilengedwe.
DTNB imagwiritsidwa ntchito makamaka poyesa kuchuluka kwa magulu a sulfhydryl (-SH) m'mapuloteni ndi ma peptide. Chosakanizacho chimagwira ntchito ndi ma thiol, zomwe zimapangitsa kuti pakhale disulfide yosakanikirana ndikutulutsa 2-nitro-5-thiobenzoate, yomwe ili ndi mtundu wachikasu womwe ungayesedwe ndi spectrophotometric. Poyesa kuchuluka kwa mtundu wachikasu, n'zotheka kuyerekeza kuchuluka kwa ma thiols mu chitsanzo. Njirayi nthawi zambiri imagwiritsidwa ntchito pophunzira momwe mapuloteni amagwirira ntchito, kupindika kwa mapuloteni, ndi machitidwe a enzymatic omwe amakhudza magulu a thiol.
Kuwonjezera pa kuwerengera kwa thiol, DTNB yagwiritsidwa ntchito m'magwiritsidwe ena, monga kuwunika momwe maselo alili, kuphunzira kukhazikika kwa mankhwala achitsulo-sulfure, komanso kuyeza ntchito ya ma enzyme osiyanasiyana.
-
PHENOLPHTHALEIN MONOPHOSPHATE BIS(CYCLOHEXYLAMMONIUM) Mchere CAS:14815-59-9
Mchere wa dicyclohexylammonium ndi mankhwala omwe amachokera ku phenolphthalein, chizindikiro cha pH chomwe chimagwiritsidwa ntchito kwambiri m'malo ochitira kafukufuku. Mchere wa dicyclohexylammonium wa phenolphthalein monophosphate nthawi zambiri umagwiritsidwa ntchito ngati chothandizira pakufufuza za biochemical ndi molecular biology. Umagwiritsidwa ntchito makamaka poyesa kapena kuyesa kuti upezeke ndikuyezera kukhalapo kwa ma enzyme ena, kuphatikizapo omwe amagwira ntchito mu ATP (adenosine triphosphate) hydrolysis kapena phosphatase activities. Kapangidwe kake kapadera kamapangitsa kuti ikhale chida chothandiza pakufufuza kwasayansi kokhudzana ndi kinetics ya enzyme ndi njira zama cell..
-
NSP-SA-NHS CAS:199293-83-9 Mtengo wa Wopanga
NSP-SA-NHS ndi mankhwala opangidwa ndi N-succinimidyl-6-(3′-pyridyldithio)hexanoate (NSP) ndi N-hydroxysuccinimide (NHS). Amagwiritsidwa ntchito kwambiri ngati cholumikizira pakati pa zochita za bioconjugation, makamaka polumikiza ma peptide, mapuloteni, kapena mamolekyu ena okhala ndi amine ku malo kapena mamolekyu ena. NSP moiety imalola kupanga mgwirizano wolimba wa thioester ndi molekyulu yomwe ikufunidwa, pomwe NHS moiety imalola kuchitapo kanthu pambuyo pake ndi ma amine oyambira. NSP-SA-NHS imagwiritsidwa ntchito kwambiri mu kafukufuku wosiyanasiyana ndi ntchito zamankhwala, kuphatikizapo kukonzekera mayeso a immunoassays, kulemba ma antibody, maphunziro okhudza kuyanjana kwa mapuloteni, ndi kuletsa kuyenda kwa mapuloteni.
-
N-(1-Naphthyl)-3-aminopropanesulfonic Acid Sodium Salt CAS:104484-71-1
Chizindikiro cha pH: Chomerachi chimasonyeza kusintha kwa mtundu kutengera pH, zomwe zimapangitsa kuti chikhale chizindikiro cha pH chothandiza m'mayeso ndi zoyeserera zosiyanasiyana. Chingagwiritsidwe ntchito poyang'anira kusintha kwa pH mu zitsanzo zamoyo, zochita za mankhwala, ndi machitidwe ena.
Chitsulo Choyezera Kuwala: N-(1-Naphthyl)-3-aminopropanesulfonic acid sodium salt ili ndi mphamvu zoyezera kuwala, zomwe zimapangitsa kuti ikhale yoyenera kuyesedwa pogwiritsa ntchito kuwala ndi kugwiritsa ntchito zilembo. Ikhoza kuphatikizidwa mu njira zowunikira kuti iwonetse ndikutsatira mamolekyulu achilengedwe kapena kapangidwe ka maselo.
Kuyesa kwa Enzyme: Kapangidwe kameneka nthawi zambiri kamagwiritsidwa ntchito ngati gawo kapena choletsa mu kuyesa kwa enzyme, makamaka zomwe zimakhudza magulu a sulfonate kapena ntchito ya enzyme yomwe imakhudzidwa ndi kusintha kwa pH. Ingathandize ofufuza kuphunzira za kinetics ya enzyme, njira zoletsa, ndi kuyanjana kwa enzyme-substrate.
Gawo la Buffer: N-(1-Naphthyl)-3-aminopropanesulfonic acid. Mchere wa sodium ungagwiritsidwe ntchito ngati gawo la machitidwe enaake a buffer, makamaka omwe amafunikira gulu la sulfonic acid kapena mitundu ina ya pH. Zimathandizira kusunga pH yabwino kwambiri m'makonzedwe oyesera.
Kupanga Mankhwala: Chifukwa cha kapangidwe kake ka mankhwala, mchere wa sodium wa N-(1-Naphthyl)-3-aminopropanesulfonic ungagwiritsidwe ntchito ngati chomangira kapena choyambira popanga mamolekyu osiyanasiyana achilengedwe kapena mankhwala.
-
N-(2-Acetamide)iminodiacetic acid CAS:26239-55-4
N-(2-Acetamido)iminodiacetic acid ndi mankhwala omwe ali m'gulu la ma amino acid omwe amadziwika kuti ma chelating agents. Amagwiritsidwa ntchito kwambiri ngati mankhwala ophatikizana, makamaka m'mafakitale ndi m'mafakitale. Mankhwalawa ali ndi mphamvu zabwino kwambiri zophatikizana ndi zitsulo, zomwe zikutanthauza kuti amatha kupanga ma complexes okhazikika ndi ma ayoni osiyanasiyana achitsulo mwa kuwagwirizanitsa kudzera m'magulu ake ogwira ntchito..
-
Disodium 4-nitrophenylphosphate CAS:4264-83-9
Disodium 4-nitrophenylphosphate ndi mankhwala okhala ndi formula ya molekyulu C6H4NO6PNa2. Ndi ufa wosalala wa kristalo womwe nthawi zambiri umagwiritsidwa ntchito mu kafukufuku wa zamoyo ndi ntchito za biology ya molekyulu. Nthawi zambiri umagwiritsidwa ntchito ngati gawo lofunikira pakuyesa ma enzyme, makamaka ma enzyme a phosphatase. Mankhwalawa amadziwika kuti amadutsa mu hydrolysis mumadzi, zomwe zimapangitsa kuti phosphate ion yaulere itulutsidwe.
-
6-methoxyquinoline CAS: 5263-87-6
6-Methoxyquinoline ndi mankhwala achilengedwe okhala ndi formula ya molekyulu C10H9NO. Ndi mankhwala a aromatic a heterocyclic omwe ali ndi mphete ya quinoline yolumikizidwa ndi gulu la methoxy pamalo a 6.
Chomera ichi chimagwiritsidwa ntchito mosiyanasiyana mu kapangidwe ka organic ndi mankhwala. Chingagwiritsidwe ntchito ngati maziko opangira zinthu zina zochokera ku quinoline ndi zinthu zina zokhudzana nazo.
Mu mankhwala, 6-Methoxyquinoline yawonetsa kuti imagwira ntchito m'mankhwala. Yaphunziridwa chifukwa cha mphamvu zake zotsutsana ndi mavairasi, malungo, komanso khansa..
-
4-Benzylamino-7-nitrobenz-2-oxa-1,3-diazole CAS:18378-20-6
4-Benzylamino-7-nitrobenz-2-oxa-1,3-diazole ndi mankhwala okhala ndi formula ya molekyulu C14H11N5O3. Ndi mankhwala ozungulira omwe ali ndi mphete ya diazole yolumikizidwa ndi mphete ya benzene. Mankhwalawa amadziwika ndi mphamvu zake zowala ndipo nthawi zambiri amagwiritsidwa ntchito ngati utoto wowala mu kafukufuku wa zamoyo ndi zithunzi. Gulu la benzyl amino lomwe limalumikizidwa ku mphete ya diazole limawonjezera kusungunuka kwake ndi kukhazikika kwake. Gulu la nitro ndi atomu ya oxa (oxygen) mu mphete ya diazole zimathandiza kuti ikhale ndi mphamvu zapadera zamakemikolo ndi zakuthupi.
